Enrichment of CD9<sup>+</sup> spermatogonial stem cells from goat (<i>Capra aegagrus hircus</i>) testis using magnetic microbeads
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منابع مشابه
Enrichment of CD9 spermatogonial stem cells from goat (Capra aegagrus hircus) testis using magnetic microbeads
The well documented source for adult multipotent stem cells is spermatogonial stem cells (SSCs) of mammalian testis. It is foundation of spermatogenesis in the testis throughout adult life by balancing self-renewal and differentiation. SSCs isolation from mammalian testis is difficult because of their scarcity and the lack of well characterized cell surface markers. Thus, the isolation of SSCs ...
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Background: This study was conducted to identify and characterize repopulating spermatogonial stem cells (SSCs) in the adult human testes. Materials and Methods: Testes biopsies from obstructive azoospermic patients and normal segments of human testicular tissue were used. Flow cytometry, real time PCR and immunohistochemical analysis were performed. Purified human spermatogonia were transplant...
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The spermatogonial stem cell initiates and maintains spermatogenesis in the testis. To perform this role, the stem cell must self replicate as well as produce daughter cells that can expand and differentiate to form spermatozoa. Despite the central importance of the spermatogonial stem cell to male reproduction, little is known about its morphological or biochemical characteristics. This result...
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Enrichment of spermatogonial stem cells (SSCs) from the mammalian adult testis faces several limitations owing to their relatively low numbers among many types of advanced germ cells and somatic cells. The aim of the present study was to improve the isolation efficiency of SSCs using a simple tissue grafting method to eliminate the existing advanced germ cells. Sliced testis parenchyma obtained...
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Mammalian spermatogenesis is a classic adult stems cell-dependent process, supported by the self-renewal and differentiation of spermatogonial stem cells (SSCs). However, the identification of SSCs and elucidation of their behaviors in undisturbed testis has long been a big challenge. Here, we generated a knock-in mouse model, Id4-2A-CreERT2-2A-tdTomato, which allowed us to mark Id4-expressing ...
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ژورنال
عنوان ژورنال: Stem Cell Discovery
سال: 2012
ISSN: 2161-6760,2161-6787
DOI: 10.4236/scd.2012.23014